R&D 1

Result of co-research with Tsukuba University
Study of functions 1st stage

Test for colorectal cancer cell growth suppression effect

Test using ES fermented soybeans

This assay uses MTT method to evaluate cancer Cell proliferation suppression

Cell used
Intestine cancer cell / Caco-2
Intestinal epithelial cell of human

Result

Suppression is concentration dependent as seen on the graph.

Mechanism

Colorectal cancer cell proliferation suppression was confirmed; mechanism of the action was evaluated

Evaluated with GUAVA-PCA
(DNA was stained to evaluate cell cycle)

Suppression is concentration dependent as seen on the graph.

Result

See the red undeline

Suppression in Cell cycle G2/M was discovered

(Reference) Features of Cell Cycle G2/M Period Suppression

Functionality Study 1st Stage Conclusion

The result of the recent study with researchers
at Tsukuba University was published in a journal of food science
Subject: "Suppression effect and its mechanism of colorectal cancer cell
proliferation by fermented soybean product"

· Han Junkyu
Tsukuba University Graduate School of Life and Envrironmental Sciences
Researcher

· Hiroko Isoda
Tsukuba University Graduate School of Life and Envrironmental Sciences
Assistant Professor

· Yukio Arai
Representative Director, Ecosunte Co., Ltd.
1992 Office Arai established
1999 Ecosunte Co, Ltd. incorporated
Appointed as the representative of the board to present